Cell cultures in trauma research


  • Risling M
  • Sondén A
  • Sköld M
  • Landegren T
  • Persson J
  • Malm E
  • Larsson I-L
  • Angeria M
  • Kjellström T

Publish date: 2003-01-01

Report number: FOI-R--1084--SE

Pages: 16

Written in: Swedish


Cell cultures can be used to elucidate biological mechanisms and for drug testing. By the use of cell cultures it has been possible to reduce the number of animals used for medical experiments. In the current report the introduction of cell cultures in trauma military medically oriented research is described. These experiments include in vitro experiments with generation of pressure waves and high power microwaves (HPM). It is concluded that cell cultures are useful for this type of research. HPM was shown to induce rapid effects on the tubulin protein in the cytoskeleton. A laser beam was used to emit a small fragment of copper which impacted the cell culture dish and genetrated a pressure wave. This model (flyer-plate) was used for studies on cytoprotection and regrowth after pressure wave injury. It is concluded that the flyer-plate model generates an injury that can be reproduced and therefore is suitable for studies on treatment after traumatic injury.