Decontamination of chemical warfare agents, such as nerve agents and mustard compunds on human skin, has been considered a major problem for many years. In recent years, the Canadian Reactive Skin Decontamination Lotion (RSDL) has been developed and is now used by several countries. This lotion has been designed for application directly on intact skin, in order to detoxify a broad spectrum of toxic agents. However, the local and systemic effects after wound decontamination with RSDL are largely unknown. The aim of the present study is to evaluate these effects. We induced a standardized deep wound in pigs by detonating a fuse on the inner thigh. Furthermore, a shallow wound was produced by removing a small piece of skin on the inner thigh. Thereafter, decontamination of these wounds and intact skin was performed. Several physiological parameters were monitored during 180 min, followed by tissue sampling and immunohistochemical staining. Analysis revealed no pathological changes in animals receiving RSDL on intact skin. Shallow wound + RSDL induced increased edema formation and a slight increase in the early inflammatory mediator c-fos, compared to animals receiving a shallow wound alone. No significant difference was observed between these groups when analysing accumulation of neutrophils. Decontamination of the deep wound caused no change in the animal's physical status. We can conclude that RSDL in wounds seems to induce some negative effects, measured as slight increase of edema and inflammation. However, these effects should not be considered sufficient to deter any medical staff from using RSDL in wounds, in a life-threatening situation.