The recreational lake Linneasjön has been the subject of numerous investigations with respect to its water quality often resulting in closure of the beaches to the public. Despite ongoing interest/investigations, important information is still missing as to what faecal sources are causing the poor water quality of the environmental water and beaches in Nybro. To further investigate the possible sources of contamination, the wetland area named Maden adjacent to Linneasjön, as well as stormwater wells were further investigated by DNA-sequencing. Bacteria associated with untreated sewage were detected in 17 out of 20 water samples in Maden, and in 11 out of 14 stormwater samples. Bacteria detected in samples taken within the Maden area were estimated, to a significant degree, to originate from stormwater, suggesting that faecal contaminations enter Linneasjön via the stormwater drainages.